Journal: Journal of colloid and interface science

Article Title: Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer.

doi: 10.1016/j.jcis.2024.06.091

Figure Lengend Snippet: Fig. 1. Proposed nanotechnological platform for targeting HER2-positive breast cancer cells. (A) Design and components of the panobinostat-loaded cubosomes functionalized with trastuzumab. (B) Panobinostat-loaded immunocubosomes administrated intravenously to treat HER2-positive breast cancer. (C) Cellular uptake, (C1) panobinostat-loaded immunocubosomes internalization by breast cancer cell line expressing HER2 (SKBR3), (C2) interaction between HER2 and trastuzumab, (C3) steps of endocytosis, and (C4) cell death.

Article Snippet: For cells, the L929 mouse fibroblast and 4T1 breast cancer were a generous gift from Prof. Dr. Jean-Christophe Leroux’s laboratory, and the SKBR3 breast cancer was purchased from DSMZ, Germany.

Techniques: Expressing

Journal: Journal of colloid and interface science

Article Title: Cubosomes functionalized with antibodies as a potential strategy for the treatment of HER2-positive breast cancer.

doi: 10.1016/j.jcis.2024.06.091

Figure Lengend Snippet: Fig. 4. Cellular uptake and cytotoxicity assessment of free Panobinostat (Free Pa), blank cubosomes (Cub), panobinostat-loaded cubosomes (CubPa) and panobinostat-loaded immunocubosomes (ICubPa) by L929, 4T1, and SKBR3 cell lines. (A) Fluorescence microscopy images of cellular uptake of Dio-loaded cubo somes and Dio-loaded immunocubosomes by L929, 4T1, and SKBR3 cell lines. The first line represents DAPI staining (nuclei, blue), the second line Dio staining (cytoplasm, green), and the third line the overlay of DAPI and Dio staining. Scale bar 50 μm. (B) Cell viability of L929, 4T1, and SKBR3 cells incubated with free panobinostat, blank cubosomes, and panobinostat-loaded cubosomes for 24 h. (C) Cell viability of L929, 4T1, and SKBR3 cells incubated with free panobinostat, panobinostat-loaded cubosomes, and panobinostat-loaded immunocubosomes for 24 h. (D) Analysis of the effectiveness of treatments by observing cell viability and cell death of SKBR3 cell incubated with free panobinostat, panobinostat-loaded cubosomes, and panobinostat-loaded imunocubosomes for 24 h. (E) Analysis of the selectivity of antibody therapy by observing cell viability and cell death of L929 and SKBR3 cells incubated with panobinostat-loaded imunocubosomes for 24 h. Data are shown as mean ± SD of at least six experiments. *Denotes statistical differences between groups, two-way ANOVA, Tukey *p < 0.05.

Article Snippet: For cells, the L929 mouse fibroblast and 4T1 breast cancer were a generous gift from Prof. Dr. Jean-Christophe Leroux’s laboratory, and the SKBR3 breast cancer was purchased from DSMZ, Germany.

Techniques: Fluorescence, Microscopy, Staining, Incubation

Journal:

Article Title: Chromosomal and Extrachromosomal Instability of the cyclin D2 Gene is Induced by Myc Overexpression

doi:

Figure Lengend Snippet: Southern blot analyses of mouse and human cell lines hybridized with murine and human cyclin D2 cDNA probes, as indicated (upper panels). Mouse lines: WEHI 231 B-cell lymphoma (low Myc) and MOPC 460D plasmacytoma (high Myc); Human lines: GL30/92T primary fibroblasts (low Myc), T47D breast carcinoma cells (high Myc), and COLO320HSR colorectal carcinoma cells (very high Myc). Digests were carried out with the enzymes indicated. 10 µg of DNA were loaded per lane. The filters were rehybridized with the cDNA of mouse R1 (lower panels) to control for amount of DNA loaded. More DNA from GL30/92T was loaded in the control wells to illustrate the restriction endonuclease products of the unamplified cyclin D2 gene. Filled arrowheads point to amplified cyclin D2 bands; empty arrowheads indicate lost bands that suggest another form of genomic instability in this tumor.

Article Snippet: Human breast ductal adenocarcinoma T47D and mouse B lymphoma WEHI 231 were obtained from the American Type Culture Collection, Rockville, MD.

Techniques: Southern Blot, Control, Amplification